Membrane compartmentalization of Ect2 and NuMA-based complexes ensure proper chromosome separation and cytokinetic furrow formation. Model for localization of Ect2/Cyk4/Mklp1 and NuMA/dynein/dynactin at the equatorial and polar membrane regions, respectively. During anaphase, the spindle midzone assembly ensures efficient localization of centralspindlin (2Cyk4:2Mklp1) together with its interacting component Ect2 enrichment at the spindle midzone and at the equatorial membrane region. On the contrary, NuMA/dynein/dynactin complexes are enriched at the spindle poles and at the polar membrane surface. Inset (i) highlights the equatorial membrane region where Ect2, because of its ability to directly associate with the membrane phosphoinositides (shown in violet), ensures constant flux of Ect2/Cyk4/Mklp1-based complexes at the equatorial membrane in the proximity of spindle midzone. This regulates sufficient levels of RhoA for proper cytokinesis. In the absence of such robust flux (for instance, in Prc1 [siRNA]), RhoA localization and cleavage furrow ingression is not affected because of the occupancy of NuMA/dynein/dynactin complexes at the polar region of the membrane (inset ii). This polarization between Ect2/Cyk4/Mklp1 and NuMA/dynein/dynactin ensures proper chromosome separation and the formation of the cytokinetic furrow. Violet and orange lipids represent phosphoinositides and other phospholipids, respectively.