Dynamics of BAF and cGAS in WT and Lmna-KO MEFs. (A) Representative immunofluorescence images of single confocal sections from the indicated MEFs fixed within 10 min after laser microirradiation and stained with anti-BANF1 (3F10-4G12) and anti-cGAS, followed with Alexa Fluor 488-labeled anti-rabbit IgG, Cy5-labeled anti-mouse IgG, and Hoechst 33342 for DNA. The right three columns are magnified views of orange boxes. (B) Dynamics of Halo-BAF and cGAS-sfCherry in the indicated MEFs. The right four columns are magnified views of orange boxes. (A and B) The rupture sites are indicated with yellow arrowheads. Bars: 5 μm (the first column) and 2 μm (the second to others).