Effect of lamin depletion on BAF and cGAS accumulation at the rupture sites. (A) Single confocal sections of WT, Lmna^−/−, Lmnb1^−/−, and Lmnb2^−/− MEFs fixed within 10 min after laser microirradiation and stained with anti-BANF1 (3F10-4G12) and anti-cGAS, followed with Alexa Fluor 488-labeled anti-rabbit IgG, Cy5-labeled anti-mouse IgG, and Hoechst 33342 for DNA. Yellow arrowheads indicate laser microirradiation-induced NE rupture sites. Blue arrowheads indicate spontaneously produced NE protrusions. Bar: 20 μm. (B and C) The max intensities of BAF (B) and cGAS (C) signals at the rupture sites. The plotted points are from two independent experiments (n = 36, 35, 32, and 34 for WT, Lmna^−/−, Lmnb1^−/−, and Lmnb2^−/−, respectively; horizontal dotted lines show the mean values, *, P < 0.05 from others by a Steel-Dwass multiple comparison). (D–F) Relative fluorescence intensities of the NLS-sfCherry in the nucleus, Halo-Baf and cGAS-sfGFP at the rupture site in WT and Lmna^−/− MEFs (means ± SEM; n = 20 cells from two independent experiments; **, P < 0.001; ns, P > 0.05 from WT by a linear mixed model). See Fig. S7 B for microscopic images.