Figure S6.
FRAP of cytoplasmic and nuclear cGAS, and the accumulation kinetics of LC, BAF and cGAS at the rupture sites. (A–D) FRAP. A 2-µm diameter spot in a nucleus or cytoplasm was bleached, and the fluorescence recovery was measured for 30 s (A and B) and 360 s (C and D; means ± SEM; n = 20 and 17 cells for B and D, respectively, from two independent experiments). (A and C) The right four columns are magnified views of orange boxes, and the photobleaching sites are indicated with yellow dotted circles. Bars: 5 μm (the first column) and 2 μm (the second to fifth column). (E) Relative intensities of sfGFP-DARPin-LA6 (top), Halo-BAF (middle), and cGAS-sfCherry (bottom) at the rupture sites in cells with cytoplasmic or nuclear cGAS in Fig. 7, A–C (means ± SEM; n = 10 cells; *, P < 0.01; **, P < 0.001 by a linear mixed model).

FRAP of cytoplasmic and nuclear cGAS, and the accumulation kinetics of LC, BAF and cGAS at the rupture sites. (A–D) FRAP. A 2-µm diameter spot in a nucleus or cytoplasm was bleached, and the fluorescence recovery was measured for 30 s (A and B) and 360 s (C and D; means ± SEM; n = 20 and 17 cells for B and D, respectively, from two independent experiments). (A and C) The right four columns are magnified views of orange boxes, and the photobleaching sites are indicated with yellow dotted circles. Bars: 5 μm (the first column) and 2 μm (the second to fifth column). (E) Relative intensities of sfGFP-DARPin-LA6 (top), Halo-BAF (middle), and cGAS-sfCherry (bottom) at the rupture sites in cells with cytoplasmic or nuclear cGAS in Fig. 7, A–C (means ± SEM; n = 10 cells; *, P < 0.01; **, P < 0.001 by a linear mixed model).

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