Figure 6.
Effect of lamin depletion on localization of BAF and cGAS. (A and B) The localization and phosphorylation of BAF in WT, Lmna−/−, Lmnb1−/−, and Lmnb2−/− MEFs was analyzed by immunofluorescence (A) and immunoblotting, respectively (B). (A) Single confocal sections of the indicated cells stained with anti-BANF1/BAF (EPR7668), followed with Alexa Fluor 488-labeled anti-rabbit IgG, and Hoechst 33342 for DNA. (B) Whole cell lysates from the indicated cells were probed with anti-LA/C, anti-LB1/2, anti-GAPDH (as loading control), and anti-BANF1/BAF (EPR7668). (C and D) The localization and phosphorylation of BAF in WT MEFs expressing scrambled control, shLA, shLC or a combination of shLA and shLC was analyzed by immunofluorescence (C) and immunoblotting, respectively (D). (C) Single confocal sections of the indicated cells stained with anti-BANF1/BAF (EPR7668), followed with Cy5-labeled anti-rabbit IgG, and Hoechst 33342 for DNA. (D) Whole cell lysates from the indicated cells were probed with anti-LA/C, anti-GAPDH (as loading control), and anti-BANF1/BAF (EPR7668). (E and F) The localization and the expression levels of cGAS in WT, Lmna−/−, Lmnb1−/−, and Lmnb2−/− MEFs was analyzed by immunofluorescence (E) and immunoblotting, respectively (F). (E) Single confocal sections of the indicated cells stained with anti-cGAS, followed with Alexa 488-labeled anti-rabbit IgG, and Hoechst 33342 for DNA. (F) Whole cell lysates from the indicated cells were probed with anti-cGAS and anti-GAPDH (as loading control). (A–F) At least two independent experiments were performed. (A, C, and E) Bars: 20 μm. (B, D, and F) Positions of the size standards are shown on the right. The values on non-phospho-BAF, phospho-BAF and LA/C are densitometric quantitation normalized to GAPDH. Source data are available for this figure: SourceData F6.

Effect of lamin depletion on localization of BAF and cGAS. (A and B) The localization and phosphorylation of BAF in WT, Lmna−/−, Lmnb1−/−, and Lmnb2−/− MEFs was analyzed by immunofluorescence (A) and immunoblotting, respectively (B). (A) Single confocal sections of the indicated cells stained with anti-BANF1/BAF (EPR7668), followed with Alexa Fluor 488-labeled anti-rabbit IgG, and Hoechst 33342 for DNA. (B) Whole cell lysates from the indicated cells were probed with anti-LA/C, anti-LB1/2, anti-GAPDH (as loading control), and anti-BANF1/BAF (EPR7668). (C and D) The localization and phosphorylation of BAF in WT MEFs expressing scrambled control, shLA, shLC or a combination of shLA and shLC was analyzed by immunofluorescence (C) and immunoblotting, respectively (D). (C) Single confocal sections of the indicated cells stained with anti-BANF1/BAF (EPR7668), followed with Cy5-labeled anti-rabbit IgG, and Hoechst 33342 for DNA. (D) Whole cell lysates from the indicated cells were probed with anti-LA/C, anti-GAPDH (as loading control), and anti-BANF1/BAF (EPR7668). (E and F) The localization and the expression levels of cGAS in WT, Lmna−/−, Lmnb1−/−, and Lmnb2−/− MEFs was analyzed by immunofluorescence (E) and immunoblotting, respectively (F). (E) Single confocal sections of the indicated cells stained with anti-cGAS, followed with Alexa 488-labeled anti-rabbit IgG, and Hoechst 33342 for DNA. (F) Whole cell lysates from the indicated cells were probed with anti-cGAS and anti-GAPDH (as loading control). (A–F) At least two independent experiments were performed. (A, C, and E) Bars: 20 μm. (B, D, and F) Positions of the size standards are shown on the right. The values on non-phospho-BAF, phospho-BAF and LA/C are densitometric quantitation normalized to GAPDH. Source data are available for this figure: SourceData F6.

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