Figure 10.
Diverse pathological agents induce lysosomal damage and SG formation response. (A) Quantification by HCM of G3BP1 and ubiquitin (Ubi) puncta in BMM cells infected with Mtb strain Erdman or its ESX-1 mutant at MOI = 10 for 20 h. White masks, algorithm-defined cell boundaries. Red masks, computer-identified G3BP1 puncta. Green masks, computer-identified ubiquitin puncta. (B) Quantification by HCM of G3BP1 and ubiquitin puncta in U2OS cells treated with FuGENE HD-coated latex beads (Polybead Amino Microsphere) for 16 h. Green masks, computer-identified G3BP1 puncta. Red masks, computer-identified ubiquitin puncta. (C) Quantification by HCM of G3BP1 and Gal3 puncta in U2OS cells treated with 1 or 10 µg/ml Tau oligomer overnight. Red masks, computer-identified G3BP1 puncta. Green masks, computer-identified Gal3 puncta. (D) Quantification by HCM of G3BP1 and ubiquitin puncta in the constructed HeLa Flp-InTetON GFP-SARS-CoV-2ORF3a cells induced by 1 µg/ml tetracycline (Tet). Red masks, computer-identified G3BP1 puncta. Pink masks, computer-identified ubiquitin puncta. Data, means ± SEM; HCM: n ≥ 3 (each experiment: 500 valid primary objects/cells per well, ≥5 wells/sample). †, P ≥ 0.05 (not significant); *, P < 0.05; **, P < 0.01, ANOVA. See also Fig. S5. Source data are available for this figure: SourceData F10.

Diverse pathological agents induce lysosomal damage and SG formation response. (A) Quantification by HCM of G3BP1 and ubiquitin (Ubi) puncta in BMM cells infected with Mtb strain Erdman or its ESX-1 mutant at MOI = 10 for 20 h. White masks, algorithm-defined cell boundaries. Red masks, computer-identified G3BP1 puncta. Green masks, computer-identified ubiquitin puncta. (B) Quantification by HCM of G3BP1 and ubiquitin puncta in U2OS cells treated with FuGENE HD-coated latex beads (Polybead Amino Microsphere) for 16 h. Green masks, computer-identified G3BP1 puncta. Red masks, computer-identified ubiquitin puncta. (C) Quantification by HCM of G3BP1 and Gal3 puncta in U2OS cells treated with 1 or 10 µg/ml Tau oligomer overnight. Red masks, computer-identified G3BP1 puncta. Green masks, computer-identified Gal3 puncta. (D) Quantification by HCM of G3BP1 and ubiquitin puncta in the constructed HeLa Flp-InTetON GFP-SARS-CoV-2ORF3a cells induced by 1 µg/ml tetracycline (Tet). Red masks, computer-identified G3BP1 puncta. Pink masks, computer-identified ubiquitin puncta. Data, means ± SEM; HCM: n ≥ 3 (each experiment: 500 valid primary objects/cells per well, ≥5 wells/sample). †, P ≥ 0.05 (not significant); *, P < 0.05; **, P < 0.01, ANOVA. See also Fig. S5. Source data are available for this figure: SourceData F10.

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