Supplemental data related to Fig. 5. (A) Analysis of Arl8b mRNA levels by quantitative PCR for RNAi-treated HeLa cells. Duplo’s measured from three independent experiments; error bar represents SEM. (B) Fluorescent analysis of Rab27a-GFP localization in HeLa overexpressing Rab5a, Rab7a, or Arl8b, as in Fig. 5 G. (C) Graphs showing quantification of number of Rab27a-GFP endosomes (left) and intensity of Rab27a-GFP endosomes (right) of small-GTPase expressing HeLa cells. n ≥ 3 images (2≥ cells per image) analyzed from ≥2 independent experiments. (D) Overlay images of Fig. 5 G, including the GTPase-RFP channels.