Figure S4.
Supplemental data related to Fig. 4. (A) Confocal analysis of ORP1L -wt, -∆ORDPHDPHD or -∆ORD (red) expression in CD63-pHluorin (green) HeLa cells, as in Fig. 4 A with single channel images in grey with inverted LUT. (B) Fluorescent analysis of VAPA-GFP (green) HeLa cells co-expressing mCherry-ORP1L-wt, -∆ORD, or ∆ORDPHDPHD (red), and labeled for endogenous CD63 (blue; inset sizes are 3.1, 3.4 and 3.4 µm, respectively). (C) Large overview of Correlative Light-Electron Microscopy (CLEM) on section on CD63-pHluorin cells transfected with ORP1L-∆ORD and post-stained with DAPI. (D) Fluorescent analysis of CD63 localization in CD63-pHluorin HeLa co-expressing HA-SKIP or HA-RILP. N, nucleus. (E) Fluorescent analysis of CD63 localization in CD63-pHluorin HeLa co-expressing GFP-SKIP, GFP-RILP, GFP-RILP-∆N, or GFP-p50 with CD63-pHuji. (F) Fusion activity of CD63-pHuji HeLa cells overexpressing GFP-RILP-∆N or GFP-p50. Control (ctrl) has filler plasmid. N, nucleus.

Supplemental data related to Fig. 4. (A) Confocal analysis of ORP1L -wt, -∆ORDPHDPHD or -∆ORD (red) expression in CD63-pHluorin (green) HeLa cells, as in Fig. 4 A with single channel images in grey with inverted LUT. (B) Fluorescent analysis of VAPA-GFP (green) HeLa cells co-expressing mCherry-ORP1L-wt, -∆ORD, or ∆ORDPHDPHD (red), and labeled for endogenous CD63 (blue; inset sizes are 3.1, 3.4 and 3.4 µm, respectively). (C) Large overview of Correlative Light-Electron Microscopy (CLEM) on section on CD63-pHluorin cells transfected with ORP1L-∆ORD and post-stained with DAPI. (D) Fluorescent analysis of CD63 localization in CD63-pHluorin HeLa co-expressing HA-SKIP or HA-RILP. N, nucleus. (E) Fluorescent analysis of CD63 localization in CD63-pHluorin HeLa co-expressing GFP-SKIP, GFP-RILP, GFP-RILP-∆N, or GFP-p50 with CD63-pHuji. (F) Fusion activity of CD63-pHuji HeLa cells overexpressing GFP-RILP-∆N or GFP-p50. Control (ctrl) has filler plasmid. N, nucleus.

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