Figure S3.
Supplemental data related to Fig. 3. (A) Western blotting analysis of cell lysates and small EVs isolated from CD63-pHluorin HeLa cells transfected with empty vector (control), Rab7a-wt, or Rab7-DN. (B) Analysis of Rab7A and protrudin mRNA levels by quantitative PCR for RNAi-treated HeLa cells. Duplo’s measured from three independent biological replicates, error bar represents standard error of the mean. (C) Western blotting analysis for ORP1L protein levels. (D) Fluorescent signal patterns for CD63-pHluorin/MagicRed in Protrudin-wt overexpressing cells, averaged over >12 events, synchronized using peak-intensity of CD63 signal at 60 s. Source data are available for this figure: SourceData FS3.

Supplemental data related to Fig. 3. (A) Western blotting analysis of cell lysates and small EVs isolated from CD63-pHluorin HeLa cells transfected with empty vector (control), Rab7a-wt, or Rab7-DN. (B) Analysis of Rab7A and protrudin mRNA levels by quantitative PCR for RNAi-treated HeLa cells. Duplo’s measured from three independent biological replicates, error bar represents standard error of the mean. (C) Western blotting analysis for ORP1L protein levels. (D) Fluorescent signal patterns for CD63-pHluorin/MagicRed in Protrudin-wt overexpressing cells, averaged over >12 events, synchronized using peak-intensity of CD63 signal at 60 s. Source data are available for this figure: SourceData FS3.

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