Figure 8.
ALK3/β3 integrin proximity induced by blue light stimulation shows the optimization of SMAD 1/5 signaling. MEFsv40 cells co-expressing Opto-CAAX-RFP/opto-β3 integrin-Venus, opto-ALK3-RFP/opto-β3 integrin-Venus, or opto-BMPRII-RFP/opto-β3 integrin-Venus were seeded onto glass before pSMAD 1/5 immunostaining. (A and C) Without sBMP2, opto-BMPR overexpression or blue light activation was not sufficient to induce the phosphorylation and translocation of SMAD 1/5 to the nucleus. (B and D) In the presence of sBMP2, the proximity between ALK3 and β3 integrin induced by blue light was able to increase SMAD 1/5 phosphorylation in the nucleus. Measurement of mean nuclear intensity: N ≥ 100 cells per condition. Unpaired t test: ****0.00001 > P. Scale bar, 100 μm.

ALK3/β3 integrin proximity induced by blue light stimulation shows the optimization of SMAD 1/5 signaling. MEFsv40 cells co-expressing Opto-CAAX-RFP/opto-β3 integrin-Venus, opto-ALK3-RFP/opto-β3 integrin-Venus, or opto-BMPRII-RFP/opto-β3 integrin-Venus were seeded onto glass before pSMAD 1/5 immunostaining. (A and C) Without sBMP2, opto-BMPR overexpression or blue light activation was not sufficient to induce the phosphorylation and translocation of SMAD 1/5 to the nucleus. (B and D) In the presence of sBMP2, the proximity between ALK3 and β3 integrin induced by blue light was able to increase SMAD 1/5 phosphorylation in the nucleus. Measurement of mean nuclear intensity: N ≥ 100 cells per condition. Unpaired t test: ****0.00001 > P. Scale bar, 100 μm.

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