Figure 2.
The spatial organization of ALK3 in adhesion sites upon BMP2 treatment depends on β3 integrin engagement with the extracellular matrix. Opto-BMPRs-RFP and opto-β3 integrin-Venus were coexpressed in MEFsv40 cells and observed by TIRF-mode imaging. (A–C) MEFsv40 cells were treated or not with sBMP2 and seeded onto (A) poly-l-lysine (PLL)-coated substrates, facilitating integrin-independent cell adhesion, and onto (B) vitronectin (VTN)- or (C) fibronectin (FN)-coated substrates to engage β3 integrin. Opto-ALK3 recruitment to adhesion sites induced by sBMP2 is dependent on integrin binding to VTN and FN. (D) Quantification of the colocalization index using Manders coefficient shows the recruitment of Opto-ALK3 to FAs upon β3 integrin binding to VTN or FN and the presence of sBMP2. N = 20 cells per condition. Scale bar, 15 µm. ***P < 0.001.

The spatial organization of ALK3 in adhesion sites upon BMP2 treatment depends on β3 integrin engagement with the extracellular matrix. Opto-BMPRs-RFP and opto-β3 integrin-Venus were coexpressed in MEFsv40 cells and observed by TIRF-mode imaging. (A–C) MEFsv40 cells were treated or not with sBMP2 and seeded onto (A) poly-l-lysine (PLL)-coated substrates, facilitating integrin-independent cell adhesion, and onto (B) vitronectin (VTN)- or (C) fibronectin (FN)-coated substrates to engage β3 integrin. Opto-ALK3 recruitment to adhesion sites induced by sBMP2 is dependent on integrin binding to VTN and FN. (D) Quantification of the colocalization index using Manders coefficient shows the recruitment of Opto-ALK3 to FAs upon β3 integrin binding to VTN or FN and the presence of sBMP2. N = 20 cells per condition. Scale bar, 15 µm. ***P < 0.001.

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