MITO-FP4 targets endogenous VASP to the mitochondria. (A) Representative confocal extended-focus projections of Raw 264.7 macrophages stained for VASP (green), transfected with the Mito-FP4 construct (red) and stained with phalloidin (blue). (B) Gallery of representative confocal projections of RAW 264.7 expressing the Lpd^EVmut construct during phagocytosis. Asterisks denote sites of phagocytic cup formation. (C) Representative confocal sections of RAW 264.7 macrophages stained with phalloidin (green) and expressing VASP (left), Mena (middle), and EVL (right). Insets represent the phagocytic cups denoted by the dotted box. Similar observations were made in three independent experimental repeats.