Figure S2.
PtdIns(3,4)P2determines the cellular localization of the PH domain of Lpd and particle association index in PtdIns(3,4)P2–depleted cells. (A) Representative confocal sections of RAW 264.7 macrophages expressing the tandem PH domain of Lpd (green) either alone or in combination with the INPP4B-CAAX construct. Similar results were observed in three independent experiments. (B) Representative confocal sections of RAW 264.7 macrophages co-expressing the tandem PH domain of Lpd (green) together with the INPP4B(C842A)-CAAX construct. Similar results were observed in three independent experimental repeats. (C) Quantification of the particle association index in in BFP-INPP4B(C842A)-CAAX (n = 178) and BFP-INPP4B-CAAX-expressing (n = 112) RAW 264.7 macrophages. Unpaired t test of individual particle association index values across N = 3 independent experiments; data are mean ± SEM. ****, P < 0.0001.

PtdIns(3,4)P 2 determines the cellular localization of the PH domain of Lpd and particle association index in PtdIns(3,4)P 2 –depleted cells. (A) Representative confocal sections of RAW 264.7 macrophages expressing the tandem PH domain of Lpd (green) either alone or in combination with the INPP4B-CAAX construct. Similar results were observed in three independent experiments. (B) Representative confocal sections of RAW 264.7 macrophages co-expressing the tandem PH domain of Lpd (green) together with the INPP4B(C842A)-CAAX construct. Similar results were observed in three independent experimental repeats. (C) Quantification of the particle association index in in BFP-INPP4B(C842A)-CAAX (n = 178) and BFP-INPP4B-CAAX-expressing (n = 112) RAW 264.7 macrophages. Unpaired t test of individual particle association index values across N = 3 independent experiments; data are mean ± SEM. ****, P < 0.0001.

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