Figure 1. PtdIns(3,4)P 2 at the plasma... | Rockefeller University Press

Figure 1.

PtdIns(3,4)P2at the plasma membrane of RAW 264.7 macrophages. (A) Confocal section of RAW 264.7 macrophages expressing GFP-cPHx2 alone (left) or in combination with either BFP-INPP4B-CAAX (middle) or BFP-INPP4B(C842A)-CAAX (right); insets represent the BFP. (B) Representative confocal micrographs of RAW 264.7 cells expressing mCherry-cPHx3 in either control (top) or 100 nM wortmannin-treated (bottom) conditions. (C) Normalized plasmalemmal mCherry-cPHx3 fluorescence intensity in control (n = 43) and wortmannin-treated cells (n = 35). For quantitation, plasmalemmal fluorescence intensity was normalized to cytosolic fluorescence intensity. Unpaired t test of individual values across n = 3 independent experiments. ****, P < 0.0001.

PtdIns(3,4)P ₂ at the plasma membrane of RAW 264.7 macrophages. (A) Confocal section of RAW 264.7 macrophages expressing GFP-cPHx2 alone (left) or in combination with either BFP-INPP4B-CAAX (middle) or BFP-INPP4B(C842A)-CAAX (right); insets represent the BFP. (B) Representative confocal micrographs of RAW 264.7 cells expressing mCherry-cPHx3 in either control (top) or 100 nM wortmannin-treated (bottom) conditions. (C) Normalized plasmalemmal mCherry-cPHx3 fluorescence intensity in control (n = 43) and wortmannin-treated cells (n = 35). For quantitation, plasmalemmal fluorescence intensity was normalized to cytosolic fluorescence intensity. Unpaired t test of individual values across n = 3 independent experiments. ****, P < 0.0001.

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