SE and TG partition nonuniformly in the core of in silico model LDs. (A) Trilayer system of MD simulations, composed of PC PLs, yellow and gray, SEs, blue, and TGs, orange. (B) Density profile of TG, SE, and PC in a trilayer system containing 50:50 TG:SE. Only a small region of the profile is shown for clarity. For density profiles, the results are reported as the average between the analysis of the first part and the last part of the simulations, while the shaded regions correspond to the area between the two curves. See Materials and methods for details. (C) Density profile of TG, SE, and PC in a trilayer system containing 25:75 TG:SE. (D) Schematic representation of interdigitation and calculations for systems at different TG:SE ratios. The results are reported as the average between the analysis of the first part and the last part of the simulations, while the error bars correspond to the distance between the results. See Materials and methods for details. (E) Interdigitation for SE and TG at different TG:SE ratios. (F) Interdigitation between PC lipids and the CHOL moiety (red) or the acyl chain (black) of SE molecules at different TG:SE ratios. (G) Representative snapshots describing the time evolution of simulations mimicking TG lipolysis into DAG. (H) Density profiles of PC, SE, TG, and DAG after equilibration. (I) Comparison of PC and SE density profile for trilayer containing 50% SE in the absence (cyan) or presence (light blue) of 30% DAG. (J) Schematic representation of shallow and deep LPDs. (K) Quantification of deep and shallow LPDs in systems with different TG:SE ratios.