DscI facilitates CortI’s cleavage furrow accumulation. (A) DIC and GFP images show localization of GFP-MyoII, GFP alone, and GFP-DscIA as cells form cleavage furrows during cytokinesis (from left to right). GFP-MyoII serves as a positive control. (B) The ratio of the background-corrected mean GFP signal intensity of both sides of the furrow (I_f) to that of both of the poles (I_p) was measured. Number of cells analyzed, n = 4–6 from three independent experiments. (C and D) GFP images show accumulation of GFP-CortI and GFP-MyoII at the cleavage furrow in cells with and without DscI. (E) Quantification of I_f/I_p ratio for GFP-CortI and GFP-MyoII signals at the cleavage furrows as cells of different genetic backgrounds divide. I_f/I_p ratio for vector expression in dsc1 cells (shaded) is reproduced from B for direct comparison. All P values were derived by ANOVA followed by Fisher’s LSD test. (F) GFP images show localization of GFP-CortI in cortI null–complemented cells with DscIA overexpression as these cells were aspirated by micropipette. Number of cells analyzed, n = 4–9 from two independent experiments. Vec, vector. (G) The degree of mechanoresponsiveness of GFP-tagged proteins was quantified as the GFP intensity ratio (I_p/I_o) of the cortex inside the pipette (I_p) to the cortex on the opposite side of the cell (I_o). P values were derived from ANOVA followed by Fisher’s LSD test. Number of cells analyzed, n = 8–9 from two independent experiments.