DscI facilitates the mechanoresponsiveness of CortI. (A) GFP images show localization of GFP-MyoII, GFP-alone, and GFP-DscIA as cells are aspirated with a micropipette (from left to right). GFP-MyoII serves as a positive control. (B) GFP images show localization of GFP-CortI in cells with and without DscI as these cells are aspirated with a micropipette. (C) GFP images show localization of GFP-MyoII in cells with and without DscI as these cells are aspirated with a micropipette. GFP-alone serves as a negative control. For A–C, the genetic background of each cell line is indicated in the upper edge of each image, and GFP-alone serves as a negative control. Micropipette suction pressure was applied to the right side of each cell. (D) The degree of mechanoresponsiveness of GFP-tagged proteins is quantified as the GFP intensity ratio (I_p/I_o) of the cortex inside the pipette (I_p) to the cortex on the opposite side of the cell (I_o). P values were derived from Kruskal Wallis followed by the Wilcoxon test. In A–D, “labeled” indicates the protein labeled by GFP, and “background” indicates the genetic background (WT or specific single or double null) expressing the labeled protein. Number of cells analyzed, n = 7–10 from four independent experiments.