The absence of DscI in the cell has no effect on the cortical localization of MyoII and IQGAP2. (A) Representative confocal immunofluorescence images of WT and dsc1 mutants stained with anti-myoII heavy chain My6 antibody (green) and Hoescht for nuclei (blue) revealed that the amount of MyoII at the cortex is unaffected in the dscI mutants. (B) Cortical MyoII localization was quantified as the ratio of mean signal intensity at the cortex (I_cort) to the mean signal intensity of the cytoplasm (I_cyt). No significant difference in this ratio between WT (n = 11) and dsc1 (n = 17) cells was present. (C) Representative DIC and GFP images of WT cells expressing GFP-alone control, iqgap2 expressing GFP-IQGAP2, and iqgap2; dsc1 expressing GFP-IQGAP2 are provided. A thin layer of IQGAP2 is present underneath the membrane in iqgap2::GFP-IQGAP2 and in iqgap2; dsc1 ::GFP-IQGAP2, but not in the GFP-alone cells. (D) Quantification of cortical IQGAP2 showed that there was no significance difference in the amount of IQGAP2 at the cortex between cells lacking DscI expression and positive control. All P values were derived for ANOVA followed by Fisher’s LSD test. Number of cells analyzed, n = 12–13.