Exogenous expression of DscIA rescues cytokinetic defects of dsc1 null mutants. (A) Western analysis verified expression of exogenous DscIA and GFP-DscIA in WT and dsc1 null background (KAx3 background). (B) Representative growth curves in suspension culture revealed that exogenous expression of DscIA rescues growth defect of dsc1 null mutants. Cell densities, as indicated, were plotted as a function of time. Growth curve for WT expressing empty plasmid is shown in black; that for dsc1 null expressing empty plasmid is shown in blue with round symbols; and that for dsc1 null expressing DscIA is shown in blue with square symbols. n = 3 per growth curve, bars represent SEM. (C) Growth rates quantified during exponential growth showed an increase in growth in dsc1 null complemented cells compared to null cells. P values were calculated using ANOVA followed by Fisher’s LSD test. (D) Quantification of the number of nuclei per cell when cells were 3 d in suspension culture. (E and F) Representative confocal immunofluorescence images of WT and dsc1 mutants stained with anti-CortI (green) and Hoescht for nuclei (blue) show that expression of DscIA in dsc1 null cells restored the level of CortI at the cortex, quantified in panel F (number of cells analyzed, n = 13–16), back to WT level. P values were derived for ANOVA followed by Fisher’s LSD test. Source data are available for this figure: SourceData FS2.