ADA and mitochondrial depolarization in EtBr-treated cells. (A) Micrographs of TMRE staining of EtBr-treated cells at varying days post-treatment or control cells treated with uridine for 10 days. For days 8 and 10 post-EtBr treatment, the images below represent increased processing to reveal the presence of cells. Scale bar: 5 μm. (B) Micrographs of actin staining (TRITC-phalloidin, green) around mitochondria (Tom20, red) at different days of EtBr treatment. DNA is stained with DAPI (blue). Images at the bottom are zooms of the boxed region. Scale bars: 5 μm. (C) Micrographs of actin staining (TRITC-phalloidin, green) around mitochondria (Tom20, red) at day 10 of EtBr treatment with or without 100 µM CK666 for 5 min before fixation. Images at the right are zooms of the boxed region. Scale bars: 10 and 5 μm.