Figure S6.
Changes in lactate production induced by mitochondrial inhibitors and hypoxia in MEFs. (A) Effect of 100 µM CK666 on lactate production upon 1 µM CCCP, 2.5 µM antimycin A, 5 µM rotenone or DMSO treatment of MEFs in 2 mM glucose without serum. Points indicate individual well measurements starting with 75,000 cells/well. n = 8 individual well measurements from four independent experiments. Statistical significance was calculated by two-way ANOVA using Tukey’s multiple comparisons test and presented in Table S1. (B) Effect of 100 µM CK666 on lactate production upon 1 µM CCCP, 2.5 µM antimycin A, 5 µM rotenone or DMSO treatment of MEFs in 25 mM glucose without serum. Points indicate individual well measurements starting with 75,000 cells/well. n = 8 individual well measurements from four independent experiments. Statistical significance was calculated by two-way ANOVA using Tukey’s multiple comparisons test and presented in Table S1. (C) Effect of 100 µM CK666 on lactate production in normoxia (21% O2) in MEFs at 2 mM glucose without serum. Points indicate individual well measurements starting with 100,000 cells/well. n = 8 individual well measurements from four independent experiments. n.s. P > 0.05; ** P = 0.002. *** P = 0.0002. Statistical significance was calculated by two-way ANOVA using Tukey’s multiple comparisons test. (D) Effect of 100 µM CK666 on lactate production in normoxia in MEFs at 25 mM glucose without serum. Points indicate individual well measurements starting with 100,000 cells/well. n = 8 individual well measurements from four independent experiments. n.s. P > 0.05. Statistical significance was calculated by two-way ANOVA using Sidak’s multiple comparisons test. (E) Effect of 100 µM CK666 on lactate production in hypoxia in MEFs at 25 mM glucose without serum. Points indicate individual well measurements starting with 100,000 cells/well. n = 8 individual well measurements from four independent experiments. n.s. P > 0.05; *** P = 0.0003. Statistical significance was calculated by two-way ANOVA using Tukey’s multiple comparisons test. Number of experiments, statistical tests, and sample sizes are provided in Table S1.

Changes in lactate production induced by mitochondrial inhibitors and hypoxia in MEFs. (A) Effect of 100 µM CK666 on lactate production upon 1 µM CCCP, 2.5 µM antimycin A, 5 µM rotenone or DMSO treatment of MEFs in 2 mM glucose without serum. Points indicate individual well measurements starting with 75,000 cells/well. n = 8 individual well measurements from four independent experiments. Statistical significance was calculated by two-way ANOVA using Tukey’s multiple comparisons test and presented in Table S1. (B) Effect of 100 µM CK666 on lactate production upon 1 µM CCCP, 2.5 µM antimycin A, 5 µM rotenone or DMSO treatment of MEFs in 25 mM glucose without serum. Points indicate individual well measurements starting with 75,000 cells/well. n = 8 individual well measurements from four independent experiments. Statistical significance was calculated by two-way ANOVA using Tukey’s multiple comparisons test and presented in Table S1. (C) Effect of 100 µM CK666 on lactate production in normoxia (21% O2) in MEFs at 2 mM glucose without serum. Points indicate individual well measurements starting with 100,000 cells/well. n = 8 individual well measurements from four independent experiments. n.s. P > 0.05; ** P = 0.002. *** P = 0.0002. Statistical significance was calculated by two-way ANOVA using Tukey’s multiple comparisons test. (D) Effect of 100 µM CK666 on lactate production in normoxia in MEFs at 25 mM glucose without serum. Points indicate individual well measurements starting with 100,000 cells/well. n = 8 individual well measurements from four independent experiments. n.s. P > 0.05. Statistical significance was calculated by two-way ANOVA using Sidak’s multiple comparisons test. (E) Effect of 100 µM CK666 on lactate production in hypoxia in MEFs at 25 mM glucose without serum. Points indicate individual well measurements starting with 100,000 cells/well. n = 8 individual well measurements from four independent experiments. n.s. P > 0.05; *** P = 0.0003. Statistical significance was calculated by two-way ANOVA using Tukey’s multiple comparisons test. Number of experiments, statistical tests, and sample sizes are provided in Table S1.

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