Line scans of ADA MEFs. (A) Micrographs of actin assembly around mitochondria in fixed MEFs, stained for actin (green) and mitochondria (red). Scale bar: 5 µm. Blue lines represent the region for line scans in B. Yellow arrows indicate punctate mitochondria without actin assembly. (B) Line scans showing the fluorescent intensity for actin and mitochondria signal across each mitochondrion as shown in A. (C) Quantification of % mitochondria displaying ADA in control (DMSO-treated) and CCCP-treated (3 min) MEFs. n = 30 cells combined from three independent experiments. Mean, SD, and number of cells given for each condition on graph. **** P < 0.0001. Statistical significance was calculated using unpaired two-tailed t tests. Number of experiments, sample sizes, and statistical tests are provided in Table S1. Video 5 shows a closedup mitochondria with ADA in U2-OS cell.