The role of Ist1 mutants, and implication of Npl4, in endosomal recycling. (A) Either WT cells or ist1∆ mutants transformed with vector (pink) or indicated Ist1 point mutations (green) were grown to mid-log phase prior to loading with dye for 8 min at RT, washing in cold media, and efflux measured by flow cytometry. (B) Table showing selected slim term annotations for recycling machinery associated with relevant enzyme activity that exhibit enrichment compared with genome-wide distribution.