Figure S1.
Differential trafficking itineraries of SNAREs and nutrient transporters. (A) Airyscan confocal microscopy of WT cells expressing an endogenously expressed Sec7-Cherry and indicated GFP-tagged proteins plasmids show expected localizations. (B) WT cells expressing GFP-Snc1 were mixed with Mup1-GFP expressing cells previously pulse-chased with FM4-64 prior to imaging. (C)rsp5-1 cells expressing Fur4-mCherry were grown to mid-log phase in SC media and processed for confocal microscopy imaging. (D) Airyscan microscopy shows colocalization and correct localization of tagged versions of Snc1 and Snc2 expressed from the CUP1 promoter. (E) Expression of GFP-Snc1 following microfluidic addition of 20 µM copper chloride to cells during time-lapse microscopy. Scale bar, 5 µM.

Differential trafficking itineraries of SNAREs and nutrient transporters. (A) Airyscan confocal microscopy of WT cells expressing an endogenously expressed Sec7-Cherry and indicated GFP-tagged proteins plasmids show expected localizations. (B) WT cells expressing GFP-Snc1 were mixed with Mup1-GFP expressing cells previously pulse-chased with FM4-64 prior to imaging. (C)rsp5-1 cells expressing Fur4-mCherry were grown to mid-log phase in SC media and processed for confocal microscopy imaging. (D) Airyscan microscopy shows colocalization and correct localization of tagged versions of Snc1 and Snc2 expressed from the CUP1 promoter. (E) Expression of GFP-Snc1 following microfluidic addition of 20 µM copper chloride to cells during time-lapse microscopy. Scale bar, 5 µM.

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