Tissue microenvironment affects EwS cell morphologies. (a–c) 10 representative cell renderings of EwS cell lines TC32 and TC71 and fibroblast cell line NIH-3T3 are shown, color-coded by k-means-based cluster from Fig. 2 e (black frames, upper part of panels). Bagplots below display PCA of each cell line as a function of region (HBV, red, PVS, black, CHT, white). The inner polygon is constructed on the basis of Tukey depth and contains at most 50% of the data points. The outer circle encompasses all data excluding outliers. White plus sign represents the Tukey depth median. Lower right panel shows a similarity matrix based on pairwise permutation tests. Note that relative to NIH-3T3 (n = 15, 22, 15 cells for HBV/PVS/CHT, respectively), which shows high similarity in morphological cell state among all three regions (a), TC32 (n = 19, 41, 47 for HBV/PVS/CHT, respectively; b) and TC71 (n = 19, 23, 18 for HBV/PVS/CHT, respectively; c) show some tissue-dependent specialization of cells. Scale bars, 20 µm.