Figure S5.
![Fis1 oligomerization regulates TBC1D15 recruitment to mitochondria. (A–C) Confocal microscopy representative images of TBC1D15 recruitment to mitochondria in HeLa cells expressing Fis1(WT) (TBC1D15, green [YFP-TBC1D15]; mitochondria, red [Tom20-mApple]). Inset in B, corresponding linescans in C. Scale bars, 5 μm (A); 1 μm (B). (D–F) Confocal microscopy representative images of TBC1D15 in the cytosol in HeLa cells expressing Fis1(LA) oligomerization mutant (TBC1D15, green [YFP-TBC1D15]; mitochondria, red [Tom20-mApple]). Inset in E, corresponding linescans in F. Scale bars, 5 μm (D); 1 μm (E).](https://cdn.rupress.org/rup/content_public/journal/jcb/221/10/10.1083_jcb.202206140/3/jcb_202206140_figs5.png?Expires=1771043222&Signature=yJV2vX14y8o7cqYAywIa-GfNjmZXjdakxx9wOfJHT4XF~kpePXe2VWI5XgfXQuv5mzFuqYvW3l76fAnksGVySXmwIIfzrVUCkOCU3uhfeomQgxD6g4FNjSc5R6tLGede4Ij7sH1PD0ywbElpFeW5ZjoUO5AW8QgD3W5isW6~3l8tUiU4GDvtyjAUoFPMU5PppbgdSOhTrYXO8C-Nws4YSzXoFtJ871TVJxbXnwWgBspVpSGf5uZ5RiMwqG5KiAzDYlnsUcV~rG-LCREeosV0X~ryk~3wl5EzD72QeHFcZ3TG~4us33ODYhxARrQag3u1vlIaNW~zZ6AkmohYJZ-IUQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fis1 oligomerization regulates TBC1D15 recruitment to mitochondria. (A–C) Confocal microscopy representative images of TBC1D15 recruitment to mitochondria in HeLa cells expressing Fis1(WT) (TBC1D15, green [YFP-TBC1D15]; mitochondria, red [Tom20-mApple]). Inset in B, corresponding linescans in C. Scale bars, 5 μm (A); 1 μm (B). (D–F) Confocal microscopy representative images of TBC1D15 in the cytosol in HeLa cells expressing Fis1(LA) oligomerization mutant (TBC1D15, green [YFP-TBC1D15]; mitochondria, red [Tom20-mApple]). Inset in E, corresponding linescans in F. Scale bars, 5 μm (D); 1 μm (E).
