PLK-1 limits the activity of GSP-2. (A) Representative images of fixed embryos of the indicated genotypes during pronuclei migration and at pronuclei centration/rotation, stained with anti-tubulin and anti–PAR-2 antibodies. Tubulin is in cyan, PAR-2 in magenta. The number of embryos analyzed is shown on the right in the pie-chart graph. N = 6. (B) PAR-2 size domain quantification of one-cell stage images from fixed embryos of the indicated genotypes once polarity is established. The quantification has been performed in embryos shown in the figure, and only the posterior PAR-2 domain was considered in the quantification. The P values were determined using the two-way ANOVA Tukey’s multiple comparisons test. N = 6. ns, P > 0.05; ****, P < 0.0001. In A and B, RNAi was performed by feeding. N = number of independent experiments.