![The L165V mutation in PAR-2 causes atypical PAR-2 cortical localization. (A) Representative midsection frames of time-lapse videos of gfp::par-2 and gfp::par-2(L165V) embryos at different cell division stages (for embryos before pronuclear migration: n = 9 and n = 14, respectively, and the other embryo stages showed: n = 14 and n = 25, respectively). The percentage on the bottom left of each image indicates the percentage of embryos with an anterior PAR-2 domain, except for the top panel (before pronuclear migration), which indicates the percentage of embryos with PAR-2 around the entire cortex. N = 4. (B) Quantification of the gfp::par-2 intensity from midsection images of live one-cell stage embryos of the indicated genotype. gfp::par-2, n = 28, gfp::par-2(L165V), n = 36, N = 2. Imaging of the gfp::par-2, used as control, has been performed the same days as the gfp::par-2(RAFA) imaging of Fig. S5 E, and it is therefore the same data. Mean is shown and error bars indicate SD. The P values were determined using two-tailed unpaired Student’s t test. (C) On the left is a representative Western blot of extracts from gfp::par-2 and gfp::par-2(L165V) embryos. Tubulin is used as a loading control. On the right, normalized ratio GFP::PAR-2/Tubulin of each experiment, N = 3. The P value (equal to 0.49 [ns]) was determined using two-tailed paired Student’s t test between the values of control and gfp::par-2(L165V) measurements. (D) Representative midsection frames of time-lapse imaging of gfp::par-2 and gfp::par-2(L165V) one-cell embryos at different cell division stages, ctrl(RNAi), n = 13 and gsp-2(RNAi), n = 16; N = 3. RNAi was performed by injection. For the top panel (before pronuclear migration) n = 6 embryos for ctrl(RNAi) and n = 8 embryos for gsp-2(RNAi). As in A, percentage on the bottom left of each embryo indicates the percentage of embryos with an anterior PAR-2 domain, with the exception of the top panel. ns, P > 0.05; *, P < 0.05. n = number of embryos analyzed; N = number of independent experiments. Source data are available for this figure: SourceData F5.](https://cdn.rupress.org/rup/content_public/journal/jcb/221/10/10.1083_jcb.202201048/2/jcb_202201048_fig5.png?Expires=1772712427&Signature=yGCxC~Evc87lTD8yFICppR4S7kA99y9wmfBlCzGynPqgONS27nzNcv~eZruHPIKdE5HCDq5SCZg6yaOKyR9S03WEp~feym5yqBCk9n7g64Xy1~aMAPPd0E4L86~ywHz8cwinkaHw6-V1yZeRMa5RVdapyWRNQaJc70ZsA0A7vg9QByCEbOsFMtLPrzD9haf7TpWRhPjYR8ZFIaIS23Qu4-AaoK3RbTIZvnFb6zXXoNnOeK04NArp2CDpGMv0lWaf7mIuHhybmyJaJ11G6OaUndn8gR12zwHfanJ1CjBW4oYXTkKB1L5kuIRM3FFxNGG~jKsAQ-ERyHtAna8IbmIpUw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The L165V mutation in PAR-2 causes atypical PAR-2 cortical localization. (A) Representative midsection frames of time-lapse videos of gfp::par-2 and gfp::par-2(L165V) embryos at different cell division stages (for embryos before pronuclear migration: n = 9 and n = 14, respectively, and the other embryo stages showed: n = 14 and n = 25, respectively). The percentage on the bottom left of each image indicates the percentage of embryos with an anterior PAR-2 domain, except for the top panel (before pronuclear migration), which indicates the percentage of embryos with PAR-2 around the entire cortex. N = 4. (B) Quantification of the gfp::par-2 intensity from midsection images of live one-cell stage embryos of the indicated genotype. gfp::par-2, n = 28, gfp::par-2(L165V), n = 36, N = 2. Imaging of the gfp::par-2, used as control, has been performed the same days as the gfp::par-2(RAFA) imaging of Fig. S5 E, and it is therefore the same data. Mean is shown and error bars indicate SD. The P values were determined using two-tailed unpaired Student’s t test. (C) On the left is a representative Western blot of extracts from gfp::par-2 and gfp::par-2(L165V) embryos. Tubulin is used as a loading control. On the right, normalized ratio GFP::PAR-2/Tubulin of each experiment, N = 3. The P value (equal to 0.49 [ns]) was determined using two-tailed paired Student’s t test between the values of control and gfp::par-2(L165V) measurements. (D) Representative midsection frames of time-lapse imaging of gfp::par-2 and gfp::par-2(L165V) one-cell embryos at different cell division stages, ctrl(RNAi), n = 13 and gsp-2(RNAi), n = 16; N = 3. RNAi was performed by injection. For the top panel (before pronuclear migration) n = 6 embryos for ctrl(RNAi) and n = 8 embryos for gsp-2(RNAi). As in A, percentage on the bottom left of each embryo indicates the percentage of embryos with an anterior PAR-2 domain, with the exception of the top panel. ns, P > 0.05; *, P < 0.05. n = number of embryos analyzed; N = number of independent experiments. Source data are available for this figure: SourceData F5.
