PAR-2 interacts with both GSP-1 and GSP-2. (A) Schematic representation of the N-terminal (1–335aa) of both wild-type PAR-2 (upper panel) and PP1 mutant (RAFA, lower panel). (B) Yeast two-hybrid assay showing the interaction between PAR-2 N (wild-type and the RAFA mutant) and GSP-2 and GSP-1. Yeasts were transformed with the indicated plasmids and were grown on selective and nonselective media. Interaction between bait and prey results in growth on selective medium (50 mM 3AT). (C) Alignment of the PP1 motif in PAR-2 for C. elegans, C. nigoni, C. briggsae, and C. remanei. Figure re-elaborated from alignment obtained with Blast (https://blast.ncbi.nlm.nih.gov).