gsp-1(RNAi) and gsp-2(RNAi) are efficient and specific for GSP-1 and GSP-2, respectively. (A) Representative midsection images of the indicated genotypes. (B) Quantification of gfp::gsp-1 levels in ctrl(RNAi; n = 25), gsp-1(RNAi) (n = 23), and gsp-2(RNAi; n = 20). N = 2. Mean is shown and error bars indicate SD. The P values were determined using ordinary one-way ANOVA. (C) Representative midsection images of the indicated genotypes. (D) Quantification of mNG::gsp-2 levels in mNG::gsp-2 after ctrl(RNAi; n = 18), gsp-2(RNAi) (n = 20), and gsp-1(RNAi) (n = 20). N = 2. Mean is shown and error bars indicate SD. The P values were determined using ordinary one-way ANOVA. RNAi was performed by feeding in all experiments shown in this figure. n = number of embryos analyzed; N = number of independent experiments. Scale bars, 5 µm. Anterior is to the left and posterior to the right. ns, P > 0.05; ****, P < 0.0001.