gsp-1(RNAi) does not rescue PAR-2 localization in the pkc-3(ne4246); gfp::par-2 strain and does not affect PAR-2 size domain in the gfp::par-2 strain. (A) Representative midsection frames of time-lapse videos of gfp::par-2 and pkc-3(ne4246); gfp::par-2 one-cell embryos at pronuclear meeting, comparing ctrl(RNAi) and gsp-1(RNAi). In gfp::par-2; ctrl(RNAi) and gfp::par-2; gsp-1(RNAi), PAR-2 localizes at the posterior cortex (n = 8 and n = 8, respectively). In pkc-3(ne4246); gfp::par-2, ctrl(RNAi) and gsp-1(RNAi) PAR-2 signal is detected uniformly at the cortex (n = 10 and n = 13, respectively). N = 3. (B) Quantification of the gfp::par-2 size domain in live zygotes at pronuclear meeting, comparing ctrl(RNAi) and gsp-1(RNAi). Numbers inside the bars indicate sample size. N = 3. The P values were determined using two-tailed unpaired Student’s t test. Mean is shown and error bars indicate SD. ns, P > 0.05. Scale bars, 5 µm. Anterior is to the left and posterior to the right. In both A and B, RNAi was performed by feeding. n = number of embryos analyzed; N = number of independent experiments.