mNG::gsp-2 and gfp::gsp-1 localize uniformly in the cytoplasm. (A) Left: representative midsection images of mNG::gsp-2 at three different stages. Right: mNG::gsp-2 intensity profile before pronuclear migration (n = 9, pink line), during pronuclear migration (n = 15, green line) and at pronuclear meeting (n = 11, orange line). N = 2. The mean is shown and error bars indicate SD. (B) Left: representative midsection images of gfp::gsp-1 at three different stages. Right: gfp::gsp-1 intensity profile before pronuclear migration (n = 9), during pronuclear migration (n = 15), and pronuclear meeting (n = 11). N = 2. Mean is shown and error bars indicate SD. n = number of embryos analyzed; N = number of independent experiments. Scale bars, 5 µm. Anterior is to the left and posterior to the right.