gsp-2(RNAi) rescues AB/P1 asymmetry and PLK-1 localization in the pkc-3(ne4246) mutant allele. (A) Quantification of cell size asymmetry (AB/P1 ratio). AB/P1 ratio equal to 1 correspond to cell symmetry, values higher or lower than 1 correspond to cell asymmetry. Numbers inside the bars indicate sample size. N = 4. Mean is shown and error bars indicate SD. The P values were determined using two-way ANOVA Tukey’s multiple comparisons test. (B) Representative two-cell stage images of fixed embryos of the indicated genotypes. DNA (DAPI) is in magenta and PLK-1 in cyan. Right: quantification of the PLK-1 levels (AB/P1 ratio) in the two-cell stage fixed embryos. Numbers inside the bars indicate sample size. N = 2. Mean is shown and error bars indicate SD. The P values were determined using two-way ANOVA Tukey’s multiple comparisons test. ns, P > 0.05; **, P < 0.01; ****, P < 0.0001. Scale bars, 5 µm. Anterior is to the left and posterior to the right. In both A and B, RNAi was performed by feeding. N = number of independent experiments.