Model of the Pan1p-mediated recruitment of endocytic proteins and actin. FKBP-tagged Pan1p makes a stable complex with End3p and becomes anchored at the peroxisome upon rapamycin addition. Pan1p recruits the mid/late coat proteins to the peroxisome and leads to actin polymerization. As actin polymerization is initiating, the peroxisome attaches to and moves on an actin cable. Once actin polymerization begins, Prk1p and actin disassembly factors, such as Cof1p and Srv2p, are recruited to the peroxisome and disassemble the coat complex and actin cytoskeleton. We posit that the same sequence of events happens at normal endocytic sites upon the recruitment of Pan1 by an as-yet-unknown triggering event.