Pan1p recruits actin cable to the peroxisome. (A and B) Localization of Abp140-3GFP and Pex-FRB-mCherry (A) after or (B) before rapamycin addition. In the upper row the left two panels show the individual channels and the right two panels show the merged image and DIC image. The time series of each protein in the boxed area is shown in the lower panels. Blue or red arrowheads indicate the timing of association or dissociation of the peroxisome and actin cable. Tracking of Pex-FRB-mCherry-labeled peroxisome in the boxed area. Green and red dots denote the first and last positions, respectively, of each peroxisome. Scale bars, 400 nm. (C) Quantification of the percentage of Pex-FRB-mCherry-labeled peroxisomes that interact with actin cables in the presence of Pan1-FKBP before and after rapamycin addition. Data show the mean ± SD from at least three experiments (n = 50 puncta for each experiment). (D) Quantification of the velocity of Pex-FRB-mCherry-labeled peroxisomes that interact with actin cables before and after rapamycin addition. Data show the mean ± SD (n = 20 puncta for each experiment). ***, P value < 0.001, unpaired t test with Welch’s correction.