Dynamic localization of endocytic proteins at Pan1p-anchoring peroxisomes. (A, C, and E) The localization of Pex-FRB-mCherry and (A) GFP-fused Las17p, (C) Myo3p, or (E) Sla2p in cells expressing Pan1-FKBP at 30 min after rapamycin addition. The upper panels show single frames from movies of the indicated strain. A time series in the boxed area is shown in the lower panels. Quantification of the normalized fluorescence intensities of Pex-FRB-mCherry and the indicated GFP-fused protein at the boxed area is shown in right. (B, D, and F) Averaged fluorescence intensities as a function of time for each protein (n = 10 independent peroxisomes). Scale bars, 2.5 μm.