Localization and fluorescence intensity of endocytic proteins at Pan1p-anchoring peroxisomes. (A) Cells expressing GFP-tagged endocytic protein, Pex-FRB-mCherry, and Pan1-FKBP were grown to log phase at 25°C, and imaged before rapamycin addition. The upper and middle images show the individual channels and the low images shows the merged overlay of the signal from both channels. (B–D) Quantification of the normalized fluorescence intensities for indicated proteins in the boxed area of Fig. 6 (A, C, and E). (E) Quantification of the normalized fluorescence intensities of Pex-FRB-mCherry, Sla1-GFP and Abp1-Turquoise2 in the boxed area of Fig. 6 G. Scale bars, 2.5 μm.