Ectopically localized Pan1p can recruit the mid- and late-coat proteins to the peroxisome. (A) Spatial-temporal recruitment of endocytic proteins. Timing of appearance and disappearance of endocytic proteins at the endocytic site are shown below. (B) Schematic diagrams of the inducible peroxisome redistribution assay. (C) Localization of GFP-tagged endocytic proteins and Pex-FRB-mCherry at 30 min after rapamycin addition. White arrowheads indicate examples of co-localization. (D) Localization of GFP-tagged endocytic proteins and Pex-FRB-mCherry at 30 min after rapamycin addition in the presence of 200 μM LatA. (E) Quantification of the percentage of the indicated GFP-tagged endocytic proteins localizing at Pex-FRB-mCherry-labeled peroxisomes before and after Rapamycin addition. Data show the mean ± SD from three experiments (n = 50 puncta for each experiment). ***, P value < 0.001, unpaired t test with Welch’s correction. Scale bars, 2.5 μm.