Las17p and Myo3p/5p cooperatively cause actin polymerization at the peroxisome. (A and E) The localization of Pex-FRB-mCherry and Abp1-GFP in cells expressing Las17-FKBP and/or Myo3/5-FKBP (A) or overexpressing Las17-FKBP (E). (B, F, and J) Quantification of the percentage of Abp1-GFP localizing at Pex-FRB-mCherry-labeled peroxisomes. Data show the mean ± SD from three experiments (n = 50 puncta for each experiment). ***, P value < 0.001, unpaired t test with Welch’s correction (F). Different letters indicate a significant difference between indicated times at P < 0.001, one-way ANOVA with Turkey’s post-hoc test (J). (C and G) A time series of the boxed area is shown in the lower panels. (D and H) Averaged fluorescence intensities as a function of time for each protein (n = 10 independent peroxisomes). (I) The localization of Pex-FRB-mCherry and Abp1-GFP in cells expressing Pan1ΔWA-FKBP, Pan1-FKBP, or Pan1-FKBP and Las17ΔWCA at 30 min after rapamycin addition.