Figure S5.
Effects of altered levels of XK on manganese accumulation in HD striatal cells. (A) Deficiency of Mn in STHdhQ111/Q111 cells. At basal and Mn-triggered states, STHdhQ111/Q111 cells enriched less Mn than STHdhQ7/Q7 cells, suggesting that HD striatal cells have a deficit in taking up Mn. (B and C) Ectopic expression of XK significantly improved STHdhQ111/Q111 cells to accumulate Mn. (D and E) However, XK-siRNA treatment had little effect on Mn accumulation in STHdhQ111/Q111 cells, though XK was efficiently lowered down upon transfection with XK-specific siRNA. Blot analyses in B and D were from one of three experiments. Data in C and E are mean ± SD. One-way ANOVA and post hoc Tukey’s analysis: F(3,8) = 1,083, P < 0.0001 (A); F(3,8) = 55.8, P < 0.001 (C); F(3,8) = 2.495, P = 0.134 (E); Tukey’s test: # P < 0.0001. Source data are available for this figure: SourceData FS5.

Effects of altered levels of XK on manganese accumulation in HD striatal cells. (A) Deficiency of Mn in STHdhQ111/Q111 cells. At basal and Mn-triggered states, STHdhQ111/Q111 cells enriched less Mn than STHdhQ7/Q7 cells, suggesting that HD striatal cells have a deficit in taking up Mn. (B and C) Ectopic expression of XK significantly improved STHdhQ111/Q111 cells to accumulate Mn. (D and E) However, XK-siRNA treatment had little effect on Mn accumulation in STHdhQ111/Q111 cells, though XK was efficiently lowered down upon transfection with XK-specific siRNA. Blot analyses in B and D were from one of three experiments. Data in C and E are mean ± SD. One-way ANOVA and post hoc Tukey’s analysis: F(3,8) = 1,083, P < 0.0001 (A); F(3,8) = 55.8, P < 0.001 (C); F(3,8) = 2.495, P = 0.134 (E); Tukey’s test: # P < 0.0001. Source data are available for this figure: SourceData FS5.

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