Figure S3.
XK is expressed normally in the striatum but is diminished on the cell surface of striatal neurons in CAG140 knock-in mice. (A and B) Postnuclear striatal and cortical supernatants of WT (N = 7) and HD (N = 6) mice were analyzed by Western blot (A) followed by densitometry (B) to examine expression levels of XK. The age of the mice was 10 mo. (C–E) A series of three consecutive coronal brain sections cut through the striatum of WT and HD140Q/140Q mice were processed for labeling with antibodies for XK with the same procedures as in Fig. 4. Immunolabeled XK molecules were detected by the avidin–biotin peroxidase method. (C) Images of immunolabeled XK in one brain section of one animal for WT and HD. Boxed regions were enlarged and shown below the corresponding photograph. (D and E) Digital images captured from the striatum of four brain sections for each genotype were analyzed with the NIH ImageJ/Fiji software to measure the cross-sectional areas (D) and signal intensity (E) of striatal neurons immunoreactive to the XK antibody. Scale bars: 150 μm. Intensities of XK, as well as GAPDH (A), immunoreactive signals were measured with the NIH ImageJ/Fiji software. Data are mean ± SD. Each symbol represents one animal (A) and one cell (D and E), respectively. Two-tailed Student’s t test was done for comparison. Source data are available for this figure: SourceData FS3.

XK is expressed normally in the striatum but is diminished on the cell surface of striatal neurons in CAG140 knock-in mice. (A and B) Postnuclear striatal and cortical supernatants of WT (N = 7) and HD (N = 6) mice were analyzed by Western blot (A) followed by densitometry (B) to examine expression levels of XK. The age of the mice was 10 mo. (C–E) A series of three consecutive coronal brain sections cut through the striatum of WT and HD140Q/140Q mice were processed for labeling with antibodies for XK with the same procedures as in Fig. 4. Immunolabeled XK molecules were detected by the avidin–biotin peroxidase method. (C) Images of immunolabeled XK in one brain section of one animal for WT and HD. Boxed regions were enlarged and shown below the corresponding photograph. (D and E) Digital images captured from the striatum of four brain sections for each genotype were analyzed with the NIH ImageJ/Fiji software to measure the cross-sectional areas (D) and signal intensity (E) of striatal neurons immunoreactive to the XK antibody. Scale bars: 150 μm. Intensities of XK, as well as GAPDH (A), immunoreactive signals were measured with the NIH ImageJ/Fiji software. Data are mean ± SD. Each symbol represents one animal (A) and one cell (D and E), respectively. Two-tailed Student’s t test was done for comparison. Source data are available for this figure: SourceData FS3.

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