Vps13c ^−/− mice do not display motor deficits or STING activation. (A) First run time (in seconds) and total number of completed runs in 60 s in the balance beam assay for Vps13c^−/− and Vps13c^−/+ mice at 6 mo old. n = 5. (B) Average time until fall over four runs in the Rotarod assay for Vps13c^−/− and Vps13c^−/+ mice at 18 mo old. n = 4. (C) Average weight of the mice used for the Rotarod assay in B. (D) qPCR of three ISG transcripts (Irf7, Zbp1, and Usp18) shows no significant difference between Vps13c^−/− and WT brain lysates from 1-yr-old mice. n = 5 biological replicates. (E) IB showing no significant difference in levels of phosphorylated STING and TBK1 between Vps13c^−/− and WT mouse brain lysates from 1-yr-old animals. n = 3 biological replicates. (F) IB showing no significant difference in levels of phosphorylated STING, TBK1, and IRF3 between Vps13c^KO and WT BMDMs under basal conditions and after treatment with 10 μM cGAMP for 24 h. n = 3 biological replicates. (G) IB showing no significant difference in levels of phosphorylated STING, TBK1, and IRF3 between Vps13c^−/− and WT fibroblasts. **, P < 0.01. Data was compared using a two-sided t test. Error bars represent ±SD. Source data associated with this figure can be found at https://doi.org/10.5281/zenodo.6416363. Source data are available for this figure: SourceData FS5.