Figure 2. Diffuse and punctate Pk1 in... | Rockefeller University Press

Figure 2.

$Diffuse and punctate Pk1 in the PCM. (A, B, D–G, and I–L) Whole PCM explants (A and B) and medium sized (D–G) and small (I–L) PCM fragments expressing Pk1-venus. Yellow arrows, Pk1 plaques; white arrows, puncta; Y, yolk platelets; yellow and white lines, positions of line plots at boundaries between Pk1-venus–labeled and unlabeled cells. Scale bars, 10 μm. (C, H, and M) Average intensities of line plots of punctate (solid) and diffuse (dotted) Pk1 from outside (left) to inside (right) of cells. Means ± SD are shown. Solid and dashed red lines indicate peak values of punctate and diffuse Pk1 in whole PCM explants. Error bars indicate SD. Whole PCM explants: punctate, n = 12; diffuse, n = 12. Medium-sized fragments: punctate, n = 16; diffuse, n = 20. Small fragments: punctate, n = 8; diffuse, n = 16. n, number of cells. (N) Fraction of small (<1 μm; n = 2 in small PCM fragments, n = 4 in medium-sized fragments, n = 37 in whole explants) and medium-sized (1–5 μm; n = 56 in small fragments, n = 93 in medium-sized fragments, n = 86 in whole explants) puncta and large plaques (>5 μm; n = 111 in small fragments, n = 52 in medium-sized fragments, n = 41 in whole explants) measured along cell membranes in PCM fragments. Counts of puncta-stained cells were pooled from experiment replicates to calculate fractions. n, number of puncta. (O) Ratio of total puncta length to cell perimeter and average number of puncta per cell in isolated cells and different-sized fragments as in N. For both measurements, n = 41 for single cells, n = 169 for small PCM fragments, n = 149 for medium-sized fragments, n = 164 for whole explants. n, number of cells. Bars indicate the mean. *, P ≤ 0.05; ****, P ≤ 0.0001 in a two-tailed Student’s t test. Data distribution was assumed to be normal but was not formally tested.$

Diffuse and punctate Pk1 in the PCM. (A, B, D–G, and I–L) Whole PCM explants (A and B) and medium sized (D–G) and small (I–L) PCM fragments expressing Pk1-venus. Yellow arrows, Pk1 plaques; white arrows, puncta; Y, yolk platelets; yellow and white lines, positions of line plots at boundaries between Pk1-venus–labeled and unlabeled cells. Scale bars, 10 μm. (C, H, and M) Average intensities of line plots of punctate (solid) and diffuse (dotted) Pk1 from outside (left) to inside (right) of cells. Means ± SD are shown. Solid and dashed red lines indicate peak values of punctate and diffuse Pk1 in whole PCM explants. Error bars indicate SD. Whole PCM explants: punctate, n = 12; diffuse, n = 12. Medium-sized fragments: punctate, n = 16; diffuse, n = 20. Small fragments: punctate, n = 8; diffuse, n = 16. n, number of cells. (N) Fraction of small (<1 μm; n = 2 in small PCM fragments, n = 4 in medium-sized fragments, n = 37 in whole explants) and medium-sized (1–5 μm; n = 56 in small fragments, n = 93 in medium-sized fragments, n = 86 in whole explants) puncta and large plaques (>5 μm; n = 111 in small fragments, n = 52 in medium-sized fragments, n = 41 in whole explants) measured along cell membranes in PCM fragments. Counts of puncta-stained cells were pooled from experiment replicates to calculate fractions. n, number of puncta. (O) Ratio of total puncta length to cell perimeter and average number of puncta per cell in isolated cells and different-sized fragments as in N. For both measurements, n = 41 for single cells, n = 169 for small PCM fragments, n = 149 for medium-sized fragments, n = 164 for whole explants. n, number of cells. Bars indicate the mean. *, P ≤ 0.05; ****, P ≤ 0.0001 in a two-tailed Student’s t test. Data distribution was assumed to be normal but was not formally tested.