Mitotic timing and fate of HCT116 cells pretreated with CENP-E inhibitor. (A) Mitotic timing of HCT116 cells. Cumulative frequencies for NEB to metaphase (NEB-Meta) and metaphase to anaphase (Meta-Ana) are shown. Cells were treated with 150 nM GSK923295 for 3 h before washout for 1 h and subsequent imaging. Control, n = 43, GSK pretreatment, n = 40; pooled from three experiments. (B) Sankey diagram to show the fate (right) of cells in each of the three metaphase classes (left). Fates include normal division, micronuclei formation, death, and other defects (lagging chromosome, cytokinesis failure). Note that the fate of cells (and not chromosomes) is tracked.