Figure S2.
Spindle assembly checkpoint and micronucleus formation in DLD-1 cells. (A) Micrographs of immunofluorescence experiments to detect Bub1 or Mad2 (SAC, green) at kinetochores (CENP-C, blue) in cells stably expressing mCherry-Sec61β (red); DAPI-stained DNA is shown in gray. Scale bars, 10 µm; 2 µm (insets). (B) Quantification of Bub1 and Mad2 immunofluorescence at kinetochores marked by CENP-C. Ensheathed chromosomes were classified using the mCherry-Sec61β signal. Dots show kinetochore measurements, boxes show IQR, bar represents the median, and whiskers show 9th and 91st percentiles (Bub1: nA = 52, nF = 49, nE = 52; (Mad2: nA = 55, nF = 57, nE = 55). (C) Stills from a video showing an example of ensheathed chromosomes in G11 DLD-1 cells forming micronuclei following Dox/IAA treatment. Scale, 10 µm.

Spindle assembly checkpoint and micronucleus formation in DLD-1 cells. (A) Micrographs of immunofluorescence experiments to detect Bub1 or Mad2 (SAC, green) at kinetochores (CENP-C, blue) in cells stably expressing mCherry-Sec61β (red); DAPI-stained DNA is shown in gray. Scale bars, 10 µm; 2 µm (insets). (B) Quantification of Bub1 and Mad2 immunofluorescence at kinetochores marked by CENP-C. Ensheathed chromosomes were classified using the mCherry-Sec61β signal. Dots show kinetochore measurements, boxes show IQR, bar represents the median, and whiskers show 9th and 91st percentiles (Bub1: nA = 52, nF = 49, nE = 52; (Mad2: nA = 55, nF = 57, nE = 55). (C) Stills from a video showing an example of ensheathed chromosomes in G11 DLD-1 cells forming micronuclei following Dox/IAA treatment. Scale, 10 µm.

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