Figure 3.
CLEM reveals that foci of exogenous SHIP164 reflect large accumulations of small vesicles. (A) SHIP164^mScarlet-positive structures juxtaposed to large GFP-WDFY2–positive endosome compartments in a COS-7 cell also expressing untagged RasG12V to induce macropinosomes. The two EM images (scale bar, 200 nm) correspond to the regions framed by two rectangles in the merge fluorescence image (scale bar, 1 µm). SHIP164 fluorescence reflects clusters of small vesicles separated from the endosomes by a band occupied by a dense matrix (arrows). (B and C) High-power view of endosomes of COS-7 cells expressing exogenous SHIP164^mScarlet (magenta), GFP-WDFY2 (green), and untagged RasG12V. Merge fluorescence images are shown at left and FIB-SEM–based reconstructions are shown at right. Scale bar, 1 µm.

CLEM reveals that foci of exogenous SHIP164 reflect large accumulations of small vesicles. (A) SHIP164^mScarlet-positive structures juxtaposed to large GFP-WDFY2–positive endosome compartments in a COS-7 cell also expressing untagged RasG12V to induce macropinosomes. The two EM images (scale bar, 200 nm) correspond to the regions framed by two rectangles in the merge fluorescence image (scale bar, 1 µm). SHIP164 fluorescence reflects clusters of small vesicles separated from the endosomes by a band occupied by a dense matrix (arrows). (B and C) High-power view of endosomes of COS-7 cells expressing exogenous SHIP164^mScarlet (magenta), GFP-WDFY2 (green), and untagged RasG12V. Merge fluorescence images are shown at left and FIB-SEM–based reconstructions are shown at right. Scale bar, 1 µm.

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