Localization of exogenous SHIP164 points to a role on endocytic organelles. (A) Live fluorescence (inverted grays) image of a COS-7 cell expressing exogenous SHIP164^mScarlet. Scale bar, 20 µm. (B) High-magnification live fluorescence images of COS-7 cells expressing exogenous SHIP164^mScarlet (magenta) and the organelle marker indicated at the top (green). The individual channels are shown as inverted grays. Scale bar, 2 µm. (C) Live image of the cytoplasm of a COS-7 cell expressing exogenous SHIP164-Halo (magenta), the endosome marker EEA1-RFP (blue), and the ER marker GFP-Sec61 (green). Scale bar, 2 µm. The individual channels are shown as inverted grays. Scale bar, 1 µm. (D) Time-series of live fluorescence images of exogenous SHIP164-mRFP (magenta) and EEA1-GFP (green). Arrowheads point to a SHIP164 accumulation undergoing fission. Time, seconds. Scale bar, 1 µm. (E) Fluorescence images of a COS-7 cell expressing exogenous SHIP164^mScarlet (magenta) and immunolabeled with antibodies against MPR (green) and GM130 (red). Scale bar, 20 µm. The zoom of individual channels are shown as inverted grays. Arrowheads indicate colocalization of exogenous SHIP164 and endogenous MPR. Scale bar, 5 µm.