Figure 3.
Apical accumulation of Rab11 vesicles depends on F-actin but not does not require intact AJs. (A, A’, B, and B’) Cytochalasin D injection (0 min) during apical constriction results in rapid diminishing of apical Rab11 vesicles without causing immediate apical myosin loss. (A) Maximum projection of apical Myosin II. Scale bar, 10 μm. (A’) Cross-section view. Yellow lines: apical surface. Scale bar, 10 μm. (B) Enface view showing apical Rab11 vesicles. Scale bar, 10 μm. (B’) Zoom-in view of B. N = 7 embryos. Scale bar, 2 μm. (C) Knockdown of junctional component α-Catenin does not prevent apical accumulation of Rab11 vesicles. N = 7 embryos for the control and eight embryos for αCat RNAi. Scale bars, 5 μm. Scale bars for zoom-in view, 2 μm.

Apical accumulation of Rab11 vesicles depends on F-actin but not does not require intact AJs. (A, A’, B, and B’) Cytochalasin D injection (0 min) during apical constriction results in rapid diminishing of apical Rab11 vesicles without causing immediate apical myosin loss. (A) Maximum projection of apical Myosin II. Scale bar, 10 μm. (A’) Cross-section view. Yellow lines: apical surface. Scale bar, 10 μm. (B) Enface view showing apical Rab11 vesicles. Scale bar, 10 μm. (B’) Zoom-in view of B. N = 7 embryos. Scale bar, 2 μm. (C) Knockdown of junctional component α-Catenin does not prevent apical accumulation of Rab11 vesicles. N = 7 embryos for the control and eight embryos for αCat RNAi. Scale bars, 5 μm. Scale bars for zoom-in view, 2 μm.

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