Figure 4.
Modulation of ROS regulates phagosomal V-ATPase recruitment during LAP. (a) Representative confocal images of ATP6V1A following 25 min OPZ stimulation of RAW264.7 cells pretreated with either DPI (5 μM) or BafA1 (100 nM). Asterisks denote phagosomes. Scale bar, 5 μm. (b) Quantification of ATP6V1A phagosome intensity from cells treated as in a. Data represent the mean of individual phagosome measurements from three independent experiments (red triangle, green square, blue circle). ****, P < 0.0001, one-way ANOVA followed by Tukey multiple comparison test. (c) Quantification of ATP6V1A phagosome intensity from RAW264.7 cells pretreated with DPI or DPI + CQ (100 μM). Data represent the mean of individual phagosome measurements from three independent experiments (red triangle, green square, blue circle). ****, P < 0.0001, one-way ANOVA followed by Tukey multiple comparison test. (d) Representative confocal images of ATP6V1A following 25 min OPZ stimulation of BMDC or BMDM cells. Asterisks denote phagosomes. Scale bar, 5 μm. (e) Quantification of ATP6V1A phagosome intensity from BMDC and BMDM cells pretreated ± DPI (5 μM). Data represent the mean of individual phagosome measurements from three independent experiments (red triangle, green square, blue circle). ****, P < 0.0001, one-way ANOVA followed by Tukey multiple comparison test.

Modulation of ROS regulates phagosomal V-ATPase recruitment during LAP. (a) Representative confocal images of ATP6V1A following 25 min OPZ stimulation of RAW264.7 cells pretreated with either DPI (5 μM) or BafA1 (100 nM). Asterisks denote phagosomes. Scale bar, 5 μm. (b) Quantification of ATP6V1A phagosome intensity from cells treated as in a. Data represent the mean of individual phagosome measurements from three independent experiments (red triangle, green square, blue circle). ****, P < 0.0001, one-way ANOVA followed by Tukey multiple comparison test. (c) Quantification of ATP6V1A phagosome intensity from RAW264.7 cells pretreated with DPI or DPI + CQ (100 μM). Data represent the mean of individual phagosome measurements from three independent experiments (red triangle, green square, blue circle). ****, P < 0.0001, one-way ANOVA followed by Tukey multiple comparison test. (d) Representative confocal images of ATP6V1A following 25 min OPZ stimulation of BMDC or BMDM cells. Asterisks denote phagosomes. Scale bar, 5 μm. (e) Quantification of ATP6V1A phagosome intensity from BMDC and BMDM cells pretreated ± DPI (5 μM). Data represent the mean of individual phagosome measurements from three independent experiments (red triangle, green square, blue circle). ****, P < 0.0001, one-way ANOVA followed by Tukey multiple comparison test.

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