Effect of E64d treatment on LAP. Confocal images of GFP-hLC3A expressing RAW264.7 cells treated with DPI (5 mM), CQ (100 mM), or E64d + pepstatin (10 μg/ml) as indicated, followed by addition of OPZ for 25 min. Asterisks denote phagosomes and arrows mark GFP-LC3A positive phagosomes. Scale bar, 5 μm.